[ In Situ Web Application ]

Welcome to the UIUC In situ hybridization gene expression website.


Quicksearch:
Gene Name 
or
Anatomy Feature 
18 dpc mouse embryo section featuring results for PCP2.  Strong expression evident in the granule cell layer of the cerebellum.
18 dpc mouse embryo section featuring results for PCP2. Strong expression evident in the granule cell layer of the cerebellum.
Human tissue array with results for Zfg1.
Human tissue array with results for Zfg1.

This site provides access to RNA in situ (ISH) gene expression data for mouse and human genes; we have also collected immunohistochemistry (IHC) data for selected proteins. We have captured gene expression patterns for a variety of genes, including candidate genes for mouse mutations and selected members of the mouse and human KRAB-zinc finger (KZNF) gene families. Another primary focus of our past studies was annotation of gene expression patterns for mouse homologs of the >1400 genes from human chromosome 19. This project was initiated as part of a joint effort to map functions for genes from this exceptionally gene-dense human chromosome, and to tie gene expression patterns with regulatory elements along the chromosome.

We hybridize digoxygenin-labeled antisense RNA probes to sectioned mouse embryos and human tissue arrays, detect hybridized RNA with fluorescent secondary labels, and scan and display the images at high resolution to permit gene expression to be examined at the single cell level. Our primary focus is capture and annotation of ISH data from sagittally sectioned 14.5 and 18.5 days post coitum (dpc) mice, providing whole-body scans of cell-type specific gene expression at two key developmental stages. Most organ systems are fully formed in 18.5 dpc mice and cell-type specific expression patterns that will persist in the adult animal are well represented at this stage for many tissues. We have also captured expression profiles of other embryonic stages for many genes. For selected genes, including lineage-specific paralogs that are not represented in mouse (for example, human KRAB zinc-finger genes, we have hybridized sectioned human tissue microarrays to capture cell-type specific gene expression patterns in a variety of adult tissues. The images are captured at high resolution using a scanning microscope and the images processed by Zoomify software to permit examine ISH data at the whole body, tissue, or cellular level. Each image has been annotated and the database can be searched by queries for specific expression patterns, by gene name, or from an ordered list of HSA19 genes. Information regarding probes and experimental conditions are also provided.

Each image has been annotated and the database can be searched by queries for specific expression patterns, by gene name, or from an ordered list of HSA19 genes. Information regarding probes and experimental conditions are also provided.


ZNF Human Database
ZNF Mouse Database